Advances in Cell Therapy for Liver Regeneration

VIEWS - 119 (Abstract) 40 (PDF)
Ling Bai, Cheng Jin


With the development of translational medicine, cell therapy has been applied in clinical practice, especially in the regenerative treatment of severe liver diseases. This article introduces the origin, development, and prospects of cell therapy from the aspects of liver disease treatment, history, current status, and clinical application feasibility of cell therapy. The article also points out the existing problems in the translational medical research of cell therapy. To achieve a favorable development in cell therapy, more fundamental and clinical are warranted.


Cell therapy, Translational medicine, Liver regeneration, Hepatocyte transplantation

Full Text:

Download PDF


Ledford H, 2008, Translational Research: The Full Cycle. Nature, 453(7197):843-5. DOI: 10.1038/453843a.

Zhang Z, Chen H, Liu D, 2011, Translational Research: Lessons From Past Research, Growing Up Nowadays, and Development Goal in Future. Sci China Life Sci, 54(12):1085-8. DOI: 10.1007/s11427-011-4252-0.

Chen FM, Jin Y, Shi ST, et al., 2011, Translational Medicine: Ten-year Overview and Future Perspectives. J Pract Stomatol, 27(1):5-11.

Guan J, Chen J, 2011, Translational Research and its Effects on Medicine in China. Chin Med J (Engl), 124(19):3170-5.

Sgroi A, Serre-Beinier V, Morel P, et al., 2009, What Clinical Alternatives to Whole Liver Transplantation? Current Status of Artificial Devices and Hepatocyte Transplantation. Transplantation, 87(4):457-66. DOI: 10.1097/tp.0b013 e3181963ad3.

Gewartowska M, Olszewski WL, 2007, Hepatocyte Transplantation-biology and Application. Ann Transplant, 12(1):27-36.

Alwahsh SM, Rashidi H, Hay DC, 2018, Liver Cell Therapy: Is this the End of the Beginning? Cell Mol Life Sci, 75(8):1307-24. DOI: 10.1007/s00018-017-2713-8.

Stravitz RT, Lee WM, 2019, Acute Liver Failure. Lancet, 394(10201):869-81. DOI: 10.1016/s0140- 6736(19)31894-x.

Crager S, 2019, Critically Ⅲ Patients with End-Stage Liver Disease. Emerg Med Clin North Am, 37(3):511-27.

Forbes SJ, Newsome PN, 2016, Liver Regeneration-mechanisms and Models to Clinical Application. Nat Rev Gastroenterol Hepatol, 13(8):473-85. DOI: 10.1038/ nrgastro.2016.97.

Cantz T, Sharma AD, Ott M, 2015, Concise Review: Cell Therapies for Hereditary Metabolic Liver Diseases-concepts, Clinical Results, and Future Developments. Stem Cells, 33(4):1055-62. DOI: 10.1002/stem.1920.

Bumgardner GL, Fasola C, Sutherland DE, 1988, Prospects for Hepatocyte Transplantation. Hepatology, 8(5):1158-61. DOI: 10.1002/hep.1840080533.

Mito M, Kusano M, Kawaura Y, 1992, Hepatocyte Transplantation in Man. Transplant Proc, 24(28):40-52.

Dhawan A, Strom SC, Sokal E, et al., 2010, Human Hepatocyte Transplantation. Methods Mol Biol, 640:525 34.

Bartlett DC, Newsome PN, 2015, Hepatocyte Cell Therapy in Liver Disease. Expert Rev Gastroenterol Hepatol, 9(10):1261-72.

Nicolas CT, Wang Y, Nyberg SL, 2016, Cell Therapy in Chronic Liver Disease. Curr Opin Gastroenterol, 32(3):189 94.

Vacanti JP, Kulig KM, 2014, Liver Cell Therapy and Tissue Engineering for Transplantation. Semin Pediatr Surg, 23(3):150-5.

Shi YJ, 2010, Central Questions Regarding the Process of Liver Regeneration. World Chin J Dig, 18(34):3611-5. DOI: 10.11569/wcjd.v18.i34.3611.

Lee CA, Sinha S, Fitzpatrick E, et al., 2018, Hepatocyte Transplantation and Advancements in Alternative Cell Sources for Liver-based Regenerative Medicine. J Mol Med (Berl), 96(6):469-81. DOI: 10.1007/s00109-018-1638-5.

Carloni G, Ponzetto A, Marchese R, et al., 2010, Liver Stem Cells and Possible Clinical Applications. Curr Stem Cell Res Ther, 5(4):314-25. DOI: 10.2174/157488810793351659.

Stieger B, Peters R, Sidler MA, et al., 2006, Hepatocyte Transplantation: Potential of Hepatocyte Progenitor Cells and Bone Marrow Derived Stem Cells. Swiss Med Wkly, 136(35-36):552-6.

Fiegel HC, Kaufmann PM, Bruns H, et al., 2008, Hepatic Tissue Engineering: From Transplantation to Customized Cell-based Liver Directed Therapies from the Laboratory. J Cell Mol Med, 12(1):56-66. DOI: 10.1111/j.1582- 4934.2007.00162.x.

Li YS, Harn HJ, Hsieh DK, et al., 2013, Cells and Materials for Liver Tissue Engineering. Cell Transplant, 22(4):685 700.

Zhang K, Zhang L, Liu W, et al., 2018, In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity. Cell Stem Cell, 23(6):806-19.

Bai RG, Muthoosamy K, Manickam S, et al., 2019, Graphene-based 3D Scaffolds in Tissue Engineering: Fabrication, Applications, and Future Scope in Liver Tissue Engineering. Int J Nanomedicine, 14:5753-83. DOI: 10.2147/ijn.s192779.

Rashidi H, Luu NT, Alwahsh SM, et al., 2018, 3D Human Liver Tissue from Pluripotent Stem Cells Displays Stable Phenotype In Vitro and Supports Compromised Liver Function In Vivo. Arch Toxicol, 92(10):3117-29. DOI: 10.1007/s00204-018-2280-2.

Kisseleva T, Gigante E, Brenner DA, 2010, Recent Advances in Liver Stem Cell Therapy. Curr Opin Gastroenterol, 26(4):395-402. DOI: 10.1097/mog.0b013e32833a6bec.

Wang Z, Li W, Jing H, et al., 2019, Generation of Hepatic Spheroids Using Human Hepatocyte-derived Liver Progenitor-like Cells for Hepatotoxicity Screening. Theranostics, 9(22):6690-705. DOI: 10.7150/thno.34520.

Nevi L, Safarikia S, Di Matteo S, et al., 2019, Hyaluronan- Based Grafting Strategies for Liver Stem Cell Therapy and Tracking Methods. Stem Cells Int, 2019:3620546. DOI: 10.1155/2019/3620546.

Wertheim JA, Baptista PM, Soto-Gutierrez A, 2012, Cellular Therapy and Bioartificial Approaches to Liver Replacement. Curr Opin Organ Transplant, 17(3):235-40. DOI: 10.1097/mot.0b013e3283534ec9.

Quante M, Wang TC, 2009, Stem Cells in Gastroenterology and Hepatology. Nat Rev Gastroenterol Hepatol, 6(12):724- 37. DOI: 10.1038/nrgastro.2009.195.

Dalgetty DM, Medine CN, Iredale JP, et al., 2009, Progress and Future Challenges in Stem Cell-derived Liver Technologies. Am J Physiol Gastrointest Liver Physiol, 297(2):G241-8. DOI: 10.1152/ajpgi.00138.2009.

Bernardo ME, Fibbe WE, 2012, Safety and Efficacy of Mesenchymal Stromal Cell Therapy in Autoimmune Disorders. Ann N Y Acad Sci, 1266:107-17. DOI: 10.1111/j.1749-6632.2012.06667.x.

Piscaglia AC, 2008, Stem Cells, A Two-edged Sword: Risks and Potentials of Regenerative Medicine. World J Gastroenterol, 14(27):4273-9. DOI: 10.3748/wjg.14.4273.

Andrewartha N, Yeoh G, 2019, Human Amnion Epithelial Cell Therapy for Chronic Liver Disease. Stem Cells Int, 2019:8106482. DOI: 10.1155/2019/8106482.

Oertel M, 2011, Fetal Liver Cell Transplantation as a Potential Alternative to Whole Liver Transplantation? J Gastroenterol, 46(8):953-65. DOI: 10.1007/s00535-011-0427-5.

Tolosa L, Pareja E, Gómez-Lechón MJ, 2016, Clinical Application of Pluripotent Stem Cells: An Alternative Cell- Based Therapy for Treating Liver Diseases? Transplantation, 100(12):2548-57. DOI: 10.1097/tp.0000000000001426.

Katsuda T, Matsuzaki J, Yamaguchi T, et al., 2019, Generation of Human Hepatic Progenitor Cells with Regenerative and Metabolic Capacities From Primary Hepatocytes. Elife, 8:e47313. DOI: 10.7554/elife.47313.

Greenhalgh SN, Matchett KP, Taylor RS, et al., 2019, Loss of Integrin αvβ8 in Murine Hepatocytes Accelerates Liver Regeneration. Am J Pathol, 189(2):258-71.

Sokal EM, 2011, From Hepatocytes to Stem and Progenitor Cells for Liver Regenerative Medicine: Advances and Clinical Perspectives. Cell Prolif, 44(1):39-43. DOI: 10.1111/j.1365-2184.2010.00730.x.

Cantz T, Manns MP, Ott M, 2008, Stem Cells in Liver Regeneration and Therapy. Cell Tissue Res, 331(1):271-82. DOI: 10.1007/s00441-007-0483-6.

Muraca M, 2011, Evolving Concepts in Cell Therapy of Liver Disease and Current Clinical Perspectives. Dig Liver Dis, 43(3):180-7. DOI: 10.1016/j.dld.2010.08.007.

Flohr TR, Bonatti H Jr., Brayman KL, et al., 2009, The Use of Stem Cells in Liver Disease. Curr Opin Organ Transplant, 14(1):64-71. DOI: 10.1097/mot.0b013e328320fd7b.

Forbes SJ, Gupta S, Dhawan A, 2015, Cell Therapy for Liver Disease: From Liver Transplantation to Cell Factory. J Hepatol, 62(1):S157-69. DOI: 10.1016/j. jhep.2015.02.040.

Fiegel HC, Kneser U, Kluth D, et al., 2010, Hepatic Tissue Engineering. Handchir Mikrochir Plast Chir, 42(6):337-41. DOI: 10.1055/s-0030-1252045.



  • There are currently no refbacks.

Copyright (c) 2019 Bai and Jin

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Recent Articles | About Journal | For Author | Fees | About Whioce

Copyright © Whioce Publishing Pte Ltd. All Rights Reserved.